Dear Hair Restoration Physician,
The 1st three lectures on the regen list were:
2 (Cole) Influence of Growth Factor Concentration and Amniotic Membrane on Dermal Papilla: Dr. John Cole (prior ref). Are higher growth factor concentrations “better” ? (e.g., 8x plt conc vs 2x, 20x plt conc vs 10x, etc.)
3 (Rose) Different PRP Systems, a Comparison: Dr. Paul Rose What are the differences in PRP prep systems? activation methods? additives (ecm, stem, etc), injection frequency?… and what is “optimal”?
Could an “optimal” system produce the “right” combination of platelet concentration + lysis + other additive(s) (stem, exosome, ecm, amniotic membrane, etc.) and
RESULT IN INCREASED HAIR GROWTH RATE? (modified hair formation animation to simulate increased hair-growth-rate treatment effect)
AND/OR INCREASED HAIR SHAFT THICKNESS? (photoshop-enlargement of dermal papilla to produce a thicker hair)
We are going to try to find out as LaserCap Company is setting up wet lab to grow hair follicles in-vitro (Philpot 2018 Raposio 2016) and our PRP Cell Lab can already produce PRP in the 2x to 20x range, along with associated platelet lysate by freeze-thaw method – e.g., links below show 10x PRP prep where we produced platelet lysate (2 million platelets down to 571k platelets after freeze-thaw; resulting growth factor concentrations in plasma to be measured by ELISA; platelet lysate plasma from 2x to 20x to be used as culture media to look for in-vitro hair length increase relative to control and/or changes in shaft thickness measure by laser micrometer (HAIRSCAN) method.
Platelet Lysate from PRP Cell Lab:
And, of course, we will test direct photomed effects on in-vitro hair growth per rx.lasercap.us as well as indirect effects by photostimulation of PRP preps prior to their in-vitro use [all part of our ‘prp + photomed for hair regrowth’ registry trial planning; see labs.lasercap.us].
Ps our PRP Cell Lab is open to perform cell counts with differentials on your PRP preparations – e.g., we can perform the same set of whole blood and prp cell counts with differentials as this $2000/prp-certification private lab, at no cost to physicians who purchase LaserCap® devices for use with their patients, and we include microscopic exam (see prp.lasercap.us; clinical relevance of microscopic to be determined, however it is an interesting visual of the prp prep).
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